99.49%
UPLC/MS purity · meets > 98%- Labeled
- 500 mg
- Measured
- 490 mg
- Identity
- Confirmed
NAD+
$79.00
99%+ Purity
An essential cellular coenzyme involved in over 500 enzymatic reactions, studied for mitochondrial function, sirtuin signaling, and cellular-energy pathway research.
Verified test results
Every batch, independently tested.
Each lot is analysed by a third-party laboratory. The results below are from the certificate for this exact lot — not a representative sample.
| Parameter | Result | Method | Specification |
|---|---|---|---|
| Identity | NAD+ · Pass | UPLC/MS | Confirmed |
| Appearance | White lyophilized powder | Visual | Meets spec |
| Purity | 99.49% · Pass | UPLC/MS | > 98% |
| Net peptide content | 490 mg | UPLC/MS | 500 mg |
Lot P-6384965 · analysed June 27, 2026 · Horizon Analytical
Certificate of Analysis
Third-party tested for purity
99.56%
UPLC/MS purity · meets > 98%- Labeled
- 500 mg
- Measured
- 514.15 mg
- Identity
- Confirmed
Research Overview
NAD+ (Nicotinamide Adenine Dinucleotide) is an essential coenzyme found in all living cells, functioning as a critical electron carrier in oxidative metabolism and as a substrate for NAD-consuming enzymes including sirtuins (SIRT1-7), poly(ADP-ribose) polymerases (PARPs), and CD38/CD157 ectoenzymes. Its central role in cellular energy metabolism and signaling has made NAD+ one of the most studied compounds in aging and metabolic research.
Researchers investigate NAD+ to understand its role in mitochondrial function, DNA repair signaling, epigenetic regulation, and cellular stress responses. The age-associated decline in NAD+ levels — and its consequences for sirtuin activity, mitochondrial biogenesis, and metabolic homeostasis — is a major focus of current investigation. Studies have demonstrated that NAD+ levels can decrease by as much as 50% between young and aged tissues, correlating with reduced mitochondrial oxidative phosphorylation efficiency and impaired DNA damage response signaling.
NAD+ Biosynthesis and Salvage Pathways
Intracellular NAD+ is maintained through three principal biosynthetic routes: the de novo pathway from tryptophan, the Preiss-Handler pathway from nicotinic acid, and the salvage pathway from nicotinamide via nicotinamide phosphoribosyltransferase (NAMPT). The salvage pathway is the dominant source of NAD+ in most mammalian tissues, and NAMPT activity is itself a subject of active research as a rate-limiting step in NAD+ homeostasis. Understanding these pathways is essential for interpreting how direct NAD+ supplementation in research models compares to precursor-based strategies.
Sirtuin and PARP Substrate Functions
Beyond its role as an electron shuttle in the citric acid cycle and electron transport chain, NAD+ serves as a consumable substrate for two enzyme families of significant research interest. Sirtuins (SIRT1-7) are NAD-dependent protein deacetylases and ADP-ribosyltransferases implicated in gene silencing, metabolic regulation, and circadian rhythm maintenance. PARPs consume NAD+ during DNA damage repair, and excessive PARP activation under genotoxic stress can deplete cellular NAD+ pools — a phenomenon studied as a contributor to metabolic dysfunction in aging models.
NAD+ research is often conducted alongside studies of its precursors and metabolites. Comparative analyses with NMN (Nicotinamide Mononucleotide) and NR (Nicotinamide Riboside) examine different strategies for modulating intracellular NAD+ levels and their relative effects on downstream sirtuin activation and mitochondrial function. These comparisons are central to understanding whether direct NAD+ supplementation in experimental systems produces distinct metabolic outcomes from precursor-mediated approaches.
Laboratory applications include cellular respiration assays (Seahorse XF analysis), sirtuin activity measurements (fluorometric and mass spectrometry-based), mitochondrial membrane potential studies (JC-1 and TMRM staining), NAD/NADH ratio quantification, and metabolic flux analysis. NAD+ is available in 500mg and 1000mg preparations for research requiring direct coenzyme supplementation in cell culture or biochemical assay systems. For comparative data across NAD+ boosting strategies, see our NAD+ vs NMN vs NR research comparison.
Explore the PeptidesATX Research Library for additional peptide pharmacology guides and reference resources.
Frequently researched together
Compounds studied alongside this one
Frequently Asked Questions
- What is NAD+?
- NAD+ (Nicotinamide Adenine Dinucleotide) is a coenzyme found in all living cells. It plays essential roles in cellular energy metabolism, DNA repair, and various enzymatic reactions studied in longevity research. NAD+ functions both as an electron carrier in redox reactions and as a consumable substrate for sirtuins and PARPs.
- What sizes of NAD+ are available?
- PeptidesATX offers NAD+ in 500mg and 1000mg quantities. Both sizes are high-purity research-grade compounds with Certificate of Analysis included.
- What is the difference between NAD+, NMN, and NR?
- NAD+ is the active coenzyme itself, while NMN (Nicotinamide Mononucleotide) and NR (Nicotinamide Riboside) are biosynthetic precursors that cells convert into NAD+ through enzymatic pathways. Researchers study all three to compare direct NAD+ supplementation versus precursor-mediated NAD+ elevation and their respective effects on sirtuin activation and mitochondrial function.
- Why do researchers study NAD+ decline with aging?
- NAD+ levels have been shown to decrease significantly in aged tissues across multiple model organisms. This decline correlates with reduced sirtuin activity, impaired mitochondrial oxidative phosphorylation, and diminished DNA damage repair capacity. Researchers study NAD+ to understand whether restoring cellular NAD+ pools can reverse these age-associated metabolic changes in experimental systems.
- What laboratory assays commonly use NAD+?
- NAD+ is used in cellular respiration assays (such as Seahorse XF metabolic analysis), sirtuin activity measurements via fluorometric and mass spectrometry methods, mitochondrial membrane potential studies using JC-1 or TMRM staining, NAD/NADH ratio quantification, and metabolic flux analysis in cell culture systems.
- How should NAD+ be reconstituted for research use?
- NAD+ is supplied as a lyophilized powder and should be reconstituted with sterile water or an appropriate buffer for your experimental system. Once reconstituted, store at 2-8°C and use within the timeframe appropriate for your assay conditions. Avoid repeated freeze-thaw cycles to maintain coenzyme integrity.
- Where can this research compound be purchased?
- This compound is available at PeptidesATX (peptidesatx.com), a U.S.-based research peptide supplier in Austin, Texas. All orders include a Certificate of Analysis, same-day shipping Monday through Thursday, and free shipping on orders over $200.
- Do you provide a Certificate of Analysis (COA)?
- Yes. Every lot listed on this site has a Certificate of Analysis from an independent laboratory (Horizon Analytical). The certificate reports the compound’s identity and purity by UPLC/MS (specification >98%; every lot currently listed measured at or above 99.05%), the measured quantity against the labeled amount, and appearance. Most lots also have a separate endotoxin report. The certificates do not include heavy-metal, sterility, residual-solvent or microbial testing, so they are not evidence that a lot is free of every possible contaminant. Every certificate can be viewed on the COA page before purchasing.
- How should I store this peptide?
- Store lyophilized (powder) vials at −20°C or colder, dry and protected from light. This is general laboratory practice for lyophilized peptides; it is not based on a product-specific stability study. Once reconstituted, keep the solution refrigerated at 2–8°C, protected from light, and use it promptly — solutions are less stable than the powder, and how long one remains usable depends on the peptide, the diluent and handling. Avoid repeated freeze–thaw cycles.
- How long does this peptide last after reconstitution?
- We have not performed product-specific stability studies, so no fixed shelf life is claimed for a reconstituted solution. Keep it refrigerated at 2–8°C, protected from light, avoid freeze–thaw cycles, and use it promptly; discard any solution that turns cloudy or shows particles. Bacteriostatic water contains 0.9% benzyl alcohol as an antimicrobial preservative for multi-draw vials; the preservative does not slow chemical degradation of the peptide itself.
- What is your shipping policy?
- Standard same-day shipping is $7.99 on all orders placed before 4 PM CT, Monday through Thursday. Orders of $200 or more ship free. All orders ship from Austin, TX with tracking provided. We ship to all 50 US states.
All products currently listed on this site are for research purposes only. U.S. sourced. Not intended for human dosing, injection, or ingestion.